Morphological Changes in Mitochondria, Mitochondrial Nucleoids, and Actin Cytoskeleton during the Transition from Anaerobic to Aerobic Culture in the Yeast <i>Saccharomyces cerevisiae</i>

We examined the morphological changes in mitochondria, mitochondrial nucleoids (mt-nucleoids), and actin cytoskeleton yeast Saccharomyces cerevisiae during transition from anaerobic to aerobic culture by immunofluorescence microscopy, DAPI staining, rhodamine-phalloidin staining. The cells grown anaerobically stationary phase contained a small number of 7.3 per cell on average. When were transferred culture, mitochondria began elongate within 1 h divided into smaller spherical 8 h. average was 29.4 at 4 h, 52.8 6 71.8 These required respiratory activity coincided well with those mt-nucleoids. In addition, we found that rapid conversion cortical patches large aggregates occurred culture.